Project

Part:BBa_K1452002:Design

Designed by: Keoni Gandall   Group: iGEM14_LA_Biohackers   (2014-11-02)


Pos/neg selection (Bacillus)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2585


Design Notes

      • the mazF toxin is unstable in plasmids without the mazE antitoxin***


Source

http://www.bgsc.org/viewdetail.php?bgscid=ECE194&Search=pDGieF http://www.ncbi.nlm.nih.gov/pubmed/16714443


References